![]() ![]() USA 80, 1169–1173.įujii-Kuriyama, Y., Mizukami, Y., Kawajiri, K., Sogawa, K., and Muramatsu, M. 57–64, North-Holland Biomedical Press, Elsevier. (1980), in Biochemistry, Biophysics and Regulation of Cytochrome P-450 (Gustafsson, J.-A., Carlstedt-Duke, J. Haniu, M., Iyanagi, T., Legesse, K., and Shively, J. Titani, K., Sasagawa, T., Resing, K., and Walsh, K. This process is experimental and the keywords may be updated as the learning algorithm improves.īöhlen, P., Stein, S., Stone, J., and Udenfriend, S. These keywords were added by machine and not by the authors. ![]() Although it is possible to perform reverse-phase HPLC using a variety of solvent systems, the trifluoroacetic acid/acetonitrile (TFA/MeCN) system in particular has become the most widely used because: (1) it has low absorbance at the 200–220 nm range (2) the recovery of peptides is in the 90–100% range (3) depending on the column and the protein, the recovery of proteins is also high (4) peak shape and resolution are superior to the non-ion-pairing systems and (5) the solvent is volatile and does not interfere with subsequent microsequence analysis. The combination of high resolution and peak sensitivity with detection of the peptide bond at 200–220 nm has made this system an attractive complement to microsequence analysis. It is well recognized that reverse-phase HPLC is one of the most powerful methods for peptide and protein isolation. ![]()
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